Camptothecin hl-60

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Web周珏，卢林明，张根葆(皖南医学院 1.病理解剖学教研室;2.病理生理学教研室;3.蛇毒研究所，安徽芜湖 241002)蛇毒具有广泛的生 WebCamptothecin is an alkaloid first isolated from the stem wood of the tree Camptotheca acuminata (Nyssaceae), and has potent antitumor activity. 31 Camptothecin acts by …

Camptothecin - immunochemistry.com

WebOct 1, 2001 · The study identifies and delineates signaling factors involved in BN80915-induced apoptosis in HL-60 cells. Cell cycle analysis of HL-60 cells treated with graded … Web14808-60-7 Wt. % 60 - 100 1 - 5 This product is a hazardous chemical as defined by the OSHA Hazard Communication Standard, 29 CFR 1910.1200. Gypsum (calcium sulfate) … people first medical supply houston tx

Identification of novel topoisomerase II alpha inhibitors by virtual ...

WebMar 7, 2000 · (1990) Diverse effects of camptothecin, an inhibitor of topoisomerase I, on the cell cycle of lymphocytic (L1210, MOLT-4) and myelogenous (HL-60, KG1) leukemic cells. Cancer Res. 50 : 5746–5750 WebDNA single- strand breaks were detected using alkaline sucrose gradient centrifugation when HL-60 cells were incubated with 10 μMCPT or 10 μMSN-38 for 30 min. These … WebNov 5, 2015 · The effect of vincristine, cisplatin or 10-hydroxy camptothecin on inducing apoptosis of HL-60 cells co-cultured with the MS-5 cell layer within 48 h was tested with/without E5. people first medical

Camptothecin - an overview ScienceDirect Topics

Effects of caspase inhibition on camptothecin‐induced …

WebOct 1, 2003 · (A) HL-60 cells were treated with 2 μM camptothecin only or in the presence of 20 μM calpeptin (calpain inhibitor), or 20 μM cathepsin inhibitor I (CI) or 20 μM ALLM (inhibits calpain and cathepsin) for the indicated times. (B) HL-60 cells were treated with 20 μM cisplatin, or 50 μM etoposide or 0.5 μg/mL adriamycin alone for 20 hours or ... WebJun 13, 2024 · Camptothecin is an anticancer molecule which acts by targeting the human deoxyribonucleic acid topoisomerase I (Topo-I). It inhibits the enzyme by blocking the re-joining step of the cleavage reaction of Topo-I, which causes accumulation of a covalent reaction intermediate. toffee colored flowersWebAug 1, 1999 · HL-60 human promyelocytic leukemia cells were cultured in RPMI 1640 supplemented with 10% fetal bovine serum. For induction of apoptosis, cells were exposed for 3 h to 0.1 μg/ml DNA topoisomerase I inhibitor, camptothecin. Quantitative analysis of apoptosis was carried out by flow cytometry. people first meaning army

"WebFounded. 1932. Founder. Harold L. Green. Defunct. 1990s. Fate. Closed as part of parent company ( McCrory Stores) bankruptcy in 2001. H. L. Green was a five and dime store … " - Camptothecin hl-60

Camptothecin hl-60

Cleavage of Bax to p18 Bax accelerates stress-induced apoptosis, …

WebCamptothecin, a cytotoxic plant alkaloid with antitumor properties, is a prototypic DNA topoisomerase I inhibitor. ... This camptothecin concentration range works well for inducing Jurkat or HL 60 cell suspensions which usually run in the 1 x 105 – 1 x 106 cell/mL concentration range. At this concentration range successful apoptosis induction ... WebCamptothecin, an quinolone alkaloid, is used as a chemotherapeutic agent in the treatment of leukemia (Jones et al., 1997). Camptothecin complexes with type I DNA …

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WebMay 1, 2000 · The promyelocytic HL-60 cell lines have been used extensively to study drug-induced apoptosis, particularly with camptothecin (Palissot et al., 1996; Shimizu and Pommier, 1997; King et al., 2000 ... WebCamptothecin is a plant alkaloid present in wood, bark, and fruit of the Asian tree Camptotheca acuminata. From a shaky beginning complicated by toxic effects and logistical issues, camptothecin derivatives have advanced to become standard components in the treatment of several malignancies.

WebAug 1, 1993 · Since apoptosis has been associated with potential involvement of intracellular signaling linked to the Ca 2+ /calmodulin and protein kinase C transduction … WebOct 1, 2003 · (A) HL-60 cells were treated with various doses of camptothecin, a DNA topoisomerase I inhibitor, for 20 hours. (B) H-7 cells were deprived of IL-3 for the indicated times. (Upper panels) Proteins were extracted and subjected to SDS-PAGE and Western blotting with anti-Bax antibody.

WebThere have been some conflicting results concerning whether annexin V binds to camptothecin (CAM)-treated HL-60 cells, a commonly used model for apoptosis. We investigated the effects of culturing HL-60 cells for up to 8 h with a range of CAM concentrations. METHODS We used flow cytometry to measure cellular light scatter, … WebJun 29, 2015 · ；D. 大黄素无法捕获Topo I-DNA 的可切割复合物，Topo 的抑制剂CPT作为阳性对照；大黄素能够诱导稳定的TopoII α-DNA 可切割复合物，Topo II 的抑制剂VP16 作为阳性对照。G. 用大黄素处理HL-60 和HL-60/MX2 细胞后，检测γ -H2AX 的表达。H. 大黄素和VP16在Topo II –缺陷型细胞HL ...

WebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s. 68 …

WebFeb 1, 2008 · So, we conclude that rhPLD2 alone cannot induce apoptosis in HL-60 cells, but it can potentiate the apoptosis of HL-60 cells induced by camptothecin. Similarly, we show that both rhPLD2 and standard PLD were able to enhance camptothecin-induced apoptosis of HL-60 cells. Previousarticlein issue Nextarticlein issue Keywords rhPLD2 … toffee color dressWebSep 1, 2001 · To shed light on the mechanism of action of hCPT at the cellular level, we compared the effects of BN80915 and the classic camptothecin SN-38, the active metabolite of irinotecan, on HL-60 human promyelocytic cancer cells. people first memphisWebThis potent antioxidant is a cytoprotectant in several experimental models. However, its neuroprotective activities are scarcely available, especially in the hippocampal region associated with... people first medical supply oklahomaWebApr 27, 2010 · Polysaccharopeptide (PSP) from Coriolus versicolor (Yunzhi) is used as a supplementary cancer treatment in Asia. The present study aims to investigate whether PSP pre-treatment can increase the response of the human leukemia HL-60 cells to apoptosis induction by Camptothecin (CPT). We used bivariate bromodeoxyuridine/propidium … people first meetingWebCamptothecin is a naturally occurring alkaloid derived from the plant Camptotheca acuminata that was identified in an anticancer drug discovery screen in the 1960s.67 … people first medical millington tnWebAug 26, 2002 · Camptothecin (CAM; Sigma, St. Louis, MO) initiates apoptosis in the human promyelocytic leukemia cell line, HL-60, via its binding to topoisomerase I–DNA … people first memphis tnWebFeb 1, 2008 · Camptothecin HL-60 cells Apoptosis Cell cycle 1. Introduction Phospholipase D (PLD) is an enzyme that hydrolyzes phospholipids. In mammalian cells, there are two PLD isoforms, PLD1 and PLD2 that share significant sequence similarity, but have distinct subcellular locations and activity. people first memphis arlington