WebMar 1, 1994 · When treated with 2 micrograms/ml cytochalasin D, both actin populations were severely disrupted and cells became flattened. Actin in the apical ring aggregated … We used an InsP3-5 phosphatase (InsP3-5P) expressed from a green fluorescent protein (GFP) polycistronic construct to investigate the implication of InsP3-mediated Ca2+ signalling in Shigella invasion25. As expected, InsP3-5P-transfected cells did not elicit whole-cell Ca2+ responses in response to relevant … See more To deplete cells from Ca2+, while limiting cytotoxic effects associated with the use of Ca2+ chelators over the length of the expriments, cells were incubated in Ca2+-free medium and … See more Transient and local Ca2+ signals, remaining confined to a small cytoplasmic region at Ca2+-releasing sites, have been described to occur independently of global Ca2+ responses, in … See more We then investigated how actin-based diffusion hindrance could shape InsP3-dependent signalling and create RATPs at Shigella-invasion … See more In other systems, the extent of diffusibility of integral membrane proteins was shown to regulate Ca2+ signalling and phagocytic processes27,28. During Shigella invasion, the unusually long … See more
ATP-Mediated Glia Signaling - Academia.edu
WebTreatment of NIH 3T3 cells with cytochalasin D (10 mM, 1 h at 37 °C) disrupted the actin cytoskeleton and changed the cells from a planar, extended morphology, to a rounded … WebThis study showed that curcumin inhibited ionomycin-induced glutamate release and KCl-evoked FM1-43 release, suggesting that some steps after Ca2+ entry are regulated by curcumin. Furthermore, disrupting the cytoskeleton organization using cytochalasin D abolished the inhibitory action of curcumin on ionomycin-induced glutamate release. hotels near buna tx
Cytochalasin - an overview ScienceDirect Topics
WebWe show here that the spatial expansion of calcium waves is mediated by ATP and subsequent activation of purinergic receptors. Ectopic expression of. Glia calcium signaling has recently been identified as a potent modulator of synaptic transmission. We show here that the spatial expansion of calcium waves is mediated by ATP and subsequent ... WebCytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Web(A) High-temporal resolution Ca 2 signals induced by glutamate (300 M), and KCl (20 mM) were recorded with a confocal microscope before and after incubation of cells with 10 M cytochalasin D ... lily kershaw songs on criminal minds