WebThe combination of omega-3 fatty acids and our unique blend of terpenes help increase the bioavailability of CBD, or the body's ability to absorb CBD, and prolong the theraputic … Web10× PCR Buffer for Blend Taq 5 μL 1× Blend Taq® (2.5U/μL) 0.5 μL 1.25 U/reaction テンプレート 1-50 ng Plasmid 10-1000 ng Genome 各プライマー (10μM) 1 μL 0.2μM each 2mM dNTPs 5 μL 0.2mM Autoclaved, distilled water to 50 μL
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WebJun 14, 2012 · The DNA concentration and purity were assessed using a spectrophotometer (NanoDrop 2000c; Thermo Scientific, Wilmington, DE, USA). The PCR test used Blend Taq Plus (Toyobo Co. Ltd. Osaka, Japan) and followed its manufacturer’s protocol except for down-scaling to 20 μl in total. WebFeb 8, 2011 · The PCR was performed in 100 μl cocktails consisting of approximately 1 ng of binary vector plasmid pBIG-ubi::GUS as a template DNA, 0.2 μM each primer, 10 μl of 10× Buffer for Blend Taq (Toyobo), 200 μM dNTP mixture and 0.725 U of Blend Taq-Plus DNA Polymerase (Toyobo). black and white timberlands
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